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For amino acid refeeding, cells were restimulated for 30 min with a 1 water-solubilized mix of essential (11130036, Thermo Fisher Scientific) and non-essential (11140035, Thermo Fisher Scientific) amino acids resuspended in amino-acid-free DMEM supplemented with 10% dialysed FBS plus glutamine

Optimization included testing the inclusion of gp42 alone or with gp350, the effect of the order of the two glycoproteins gH and gL in tandem expression constructs, the timing and dosing of repRNA delivery, the effect of soluble versus membrane-anchored antigen, and the delivery of gH/gL fused to different multimeric constructs encoded by repRNA

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For example, miR-7-5p inhibits ferroptosis by downregulating mitoferrin and reducing iron levels in radio-resistant cells (Tomita et al., 2019)

The answer to whether GHK-Cu topical or injection method is the right one for you should be clearer by now