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For the dot blots, the protein concentration was normalized using BCA (Fisher Scientific, #PI23227) and 3g in 3L was spotted onto nitrocellulose membrane (Bio-Rad, #1620115)

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Here are some of them: Improper or poor hair removal techniques (excessive shaving or long-term use of depilatory creams that irritate the skin) Accumulation of dead skin cells in the area The use of antiperspirant and deodorants Hyperpigmentation Friction due to tight clothes Hormonal imbalance (during pregnancy, menstruation, or having Polycystic Ovary Syndrome) Do take note that this darkening of the skin can also be hereditary

These primed SCs convert most glucose to lactate, facilitating the recycling of NAD from the cytosol and ensuring their utilization in other cellular synthesis pathways