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10.3390/ijms232113000 27 FukuchiK.SakagamiH.IkedaM.KawazoeY.Oh-haraT.KonnoK.et al (1989)

Evaluation ofex vivocardiac function After decapitation, an emergency thoracotomy was performed, and rat hearts were isolated, attached via an aortic cannula, and retrogradely perfused using the Langendorff technique at a gradually increasing perfusion pressure between 40 120 cm H 2 O [12]

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Importantly, the influence of the gutvagina axis extends beyond local vaginal health and involves broader regulatory pathways, including hormonal modulation, systemic metabolic regulation, and immune responses 346

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