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Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method

The cell viability was calculated from the absorbance at 650 nm (derived from neutral red) and 550 nm (derived from cells themselves)

We also use advanced imaging or genetic testing

The activation of PI3K, one of the mutated genes in many solid tumors [252, 253], could trigger the PI3K/Akt/mTOR signaling pathway, inhibiting functional performance of cytotoxic T cells and decreasing tumor infiltration of immune cells, eventually causing resistance to T cells-mediated immunotherapy [254]

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