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Perry, D

DNA dot-blot analysis Genomic DNA from erythroblasts was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated with ethanol before dissolving in water

U-87 MG cells were harvested from a human glioblastoma tumor, and although it is in their inherent nature to cluster when they are cultured in standard 2D culture, they adopt fibroblast-like morphology and only make small aggregates (Fig 1A)