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Real time quantitative PCR Total RNA was obtained from 1.5 mL of aliquots from endpoints Z, A and B, using the QIAamp RNA Blood kit (Qiagen, Hilden, Germany), and cDNA synthesis was performed from 2 g of total RNA using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher, Waltham, Massachusetts), both according to manufacturers protocols

Beef, poultry, and wild-caught fish are excellent sources of methionine and cysteine

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Injectable decellularized nucleus pulposus-based cell delivery system for differentiation of adipose-derived stem cells and nucleus pulposus regeneration

The above results suggest that different doses, frequency of administration, or method of administration of capsaicin may be the reasons for the differences in conclusions