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The secondary antibodies used were anti-rabbit Alexa Fluor 647 (1:250, 711605-152, Jackson ImmunoResearch), anti-Chicken Alexa Fluor 647 (1:250, 703605-155, Jackson ImmunoResearch), anti-Rabbit Dylight 405 (1:250, 711475, Jackson ImmunoResearch), and anti-Mouse Alexa Fluor 647 (1:250, 715605-150, Jackson ImmunoResearch)