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For the apoptosis test, the spheroids were treated with PBS, Ru(II)-OH, NP2, or NP4 for 24 h, respectively (Ru 10 M)

ROS-mediated cell signaling As outlined above, ROS have origin from two main sources: mitochondrial oxidative metabolism and processes of cellular response to xenobiotics, bacteria, and cytokines during which ROS are formed as a part of either signal transduction pathways or a cellular defense mechanism (Finkel 2011)

10.1021/jf803011r

3.2 Advantages and limitations of the assays 3.2.1 ORAC The ORAC assay facilitates high-throughput screening through microplate-based parallel analysis

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