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Beads were sequentially washed in 500-l solutions of: 1x 1% SDS in 50 mM HEPES, 1x 500 l 0.2% SDS in 50 mM HEPES, 1x 500 l 0.2% SDS, 40 mM CAA, 10 mM TCEP in 50 mM HEPES (10-min incubation with agitation) and finally 2x 500 l 50 mM HEPES

doi:10.1021/tx3000494 Cuddihy SL, Parker A, Harwood DT, Vissers MC, Winterbourn CC (2008) Ascorbate interacts with reduced glutathione to scavenge phenoxyl radicals in HL60 cells