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GSNO was used solely as a nitrosylating agent to generate S-nitrosylated TTR, as previously shown [64], and excess reagent was removed prior to analysis

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Zinc: modulating inflammation, androgen production, and insulin resistance Zinc is an important trace mineral that is involved in many different physiological functions, including insulin production, oxidative stress responses, and regulating reproductive hormones

In all assays the reactions were performed in 1 ml 0.1 M potassium phosphate (pH 7.4), 10 mM EDTA using 100 M NADPH and 20 nM TGR