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concluded that in a rat supraspinatus overuse tendinopathy model, exogenous melatonin administration (10 mg/kg twice daily) significantly reduced oxidative stress, iNOS and VEGF levels, improving the oxidant-antioxidant balance (lower OSI, TOS, and VEGF) and attenuating inflammation comparably to corticosteroid therapy (Kocadal et al., 2019)

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These cells were then mounted in ProLong Glass antifade mounting medium (Life Technologies, USA), followed by treatment with fluorophore-conjugated goat anti-mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 and goat anti-rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 antibody and subsequent washing