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The following day, guinea pig anti-rabbit secondary antibody was bound to primary antibodies, followed by an incubation with assembled pA-Tn5 transposomes

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Direct immunomodulatory effects on CD4 + T lymphocytes, enhancing regulatory T cell (Treg: CD4 + , CD25 + , FoxP3 + ) differentiation while suppressing Th1-mediated gonadal WAT (gWAT) inflammation