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Description

GLI1-expressing mesenchymal cells form the essential Wnt-secreting niche for colon stem cells

Vitamin D is particularly important for psoriasis

Samples were then incubated in the appropriate primary antibodies in PBS-T with 10% fetal bovine serum, followed by three rinses in PBS-T and labelling with AlexaFluor-conjugated secondary antibodies (1:250, Life Technologies) in PBS-T for 1 hour at room temperature