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The change in the DNA causes the enzyme to be more thermolabile, which means that the enzyme loses its activity when exposed to moderate heat

10.1007/s00018-016-2194-1 16 CaoY.LiY.HeC.YanF.LiJ.-R.XuH.-Z.et al (2021)

7.4 Osmolality Approx

We introduced Dox-inducible FL Flag-tagged p21, or isolated N and C terminal truncation mutants (p21 N and p21 C , respectively), into HT-1080 p21 KO cells (Tarangelo et al, 2018)