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Multiplex immunohistochemistry (mIHC) staining The mIHC staining was performed with Opal polaris 7 color automation IHC detection kit (AccuraMed) following the manufacturers instructions

111 Erastin increases Fe 2+ levels and ROS production in breast cancer cells, promoting ferroptosis

Ratiometric imaging of the fluorescence emission at the indicated excitation wavelengths allows for the calculation of the ratio of reduced to oxidized glutathione (GSH/GSSG)