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Assessment of reduced Trx1 or Grx1 in vitro Trx1 and Grx1 were reduced with 10 mM dithiothreitol (DTT) for 1 hour at 37 C, and desalted using a NAP-5 column (GE Healthcare, Uppsala, Sweden) and eluted with TE buffer for Trx1 or KE buffer for Grx1

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