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Finkelstein J, Martin J: Methionine metabolism in mammals: distribution of homocysteine between competing pathways

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One way, two way or repeated ANOVA and appropriate post-hoc tests were used to identify significant differences, with a significance level set at p 0.9999)

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After this, the gelling chambers were removed, and specimens were incubated in 8 U/mL proteinase K (Sigma-Aldrich P2308) in a Tris/EDTA-based digestion buffer (50 mM Tris (pH 8), 25 mM EDTA, 0.5% Triton X-100 and 0.8 M NaCl) at 60C for 4 h