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Cell lysates and tissue homogenates were denatured by incubation with Laemmli sample buffer (5.1% glycerol, 0.51 % SDS, 0.051% bromophenol blue, 30 mM DTT, 10.6 mM Tris-HCl, pH 6.9) for 5 min at 95 C

To establish a DTXresistant subline, a multiphase stepwise selection protocol was applied over an 8week period

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however, human data remain limited and inconsistent

The activity of GR (EC 1.6.4.2) was measured by observing decreased absorbance at 340 nm for GSSG-dependent oxidation of NADPH by using the extinction coefficient of 6.2 mM 1 cm 1 49