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Sdha detection was performed on FoB from total spleen with the MitoBiogenesis Flow Cytometry Kit (Abcam ab168540) following the manufacturers instructions

They have a slow onset of action for producing clinical effects (weeks to months )

Although spatial multiomics and single-cell omics techniques can provide a deeper understanding of tissue and cellular heterogeneity, elucidation of the molecular and phenotypic heterogeneity in disease pathways underlying diabetic complications and the complex interplay of risk factors, such as obesity, aging, and inflammation, poses significant challenges to the discovery of biomarkers and the development of standardized therapeutic strategies

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Therefore, to determine if the tag fusion is having an impact on the activity of the target protein, complementation studies to monitor the wild-type versus the recombinant proteins activities would be required