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Androgen receptor increases hematogenous metastasis yet decreases lymphatic metastasis of renal cell carcinoma

Materials and methods Media and culture conditions Escherichia coli DH5 strains were grown at 37 C in LB medium (1% tryptone, 0.5% yeast extract, 1% NaCl w/v) supplied with 50 g/mL kanamycin or 100 g/mL ampicillin (Sigma-Aldrich, Missouri, USA) for cloning and plasmid propagation

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The reaction solution was dialyzed against DIW and lyophilized to purify and collect the 3-PLG-A-GSH

Key Research Applications Peptide Reconstitution: Dissolves lyophilized peptide powder into a stable injectable solution for research dosing studies and pharmacokinetic work