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SARS-CoV-2-infected patients with COVID-19 disease show alterations in the glucoseinsulin axis leading to hyperglycemia, hyperinsulinemia and insulin resistance, increased oxidative/nitrosative stress, and significantly decreased vitamin D, thiols, total-antioxidant-capacity, GSH and selenium (Soto et al., 2022)

In the current study, depriving RPE cells of the essential GSH precursor Cys 2 by culturing cells in Cys 2 -free culture medium or blocking de novo synthesis of GSH by using GCL inhibitor BSO or blocking Cys 2 uptake by pharmaceutical inhibition of system x c caused cell death

2019;3(11):nzz108

coli gshA , dsbA double mutant, the kanamycin cassette was removed from the gshA KEIO mutant using the plasmid 709-FLPE as indicated by the supplier (Gene Bridges, Heidelberg, Germany)