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Description

Co-immunoprecipitation (co-IP) Co-IP experiments were performed as previously described 49

10.1007/978-1-4939-7201-2_18 134 KrasoulisA.AntonopoulosN.PitsikalisV.TheodorakisS

Here we establish a human primary culture system that allows sensitive discrimination of cell fate dynamics and apply single-cell CRISPR interference (CRISPRi) screening 13,14 to examine the transcriptional and cell fate consequences of 44 TFs active during cortical neurogenesis