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doi: 10.1016/j.pharmthera.2017.05.002 83 ZhangWXiaoDMaoQXiaH

sgRNA-expressing mCherry + cells were then sorted into the brightest and dimmest 30% GFP + populations in 15-ml conical flasks containing DMEM with 4.5 g L 1 glucose and L-glutamine supplemented with 10% FBS