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the initial value of was set to the constant estimated from linear interpolation by linear regression between E1 and E5, obtained from the estimate of TST of all 15 participants (i.e., the estimated number of days until the difference between E1 and E5 showed a decrease of 37% (2.52))

We further investigated whether Ggt1-induced glutamate and its products perturbed downstream pathways

Injecting into wet alcohol stings because the alcohol gets carried under the skin by the needle

Notes: Clear guidance, patch test, avoid over-layering

For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C