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10.1016/j.celrep.2020.01.011 63

Measurement of protein carbonylation was conducted by using Protein Carbonyl Assay Kit (ab178020, Abcam Co

Well-designed preclinical and clinical trials are urgently needed

Multiple sequence alignments and sequence logos A multiple protein sequence alignment of known arGSTs of the phi class ( Ph AN9 28 (O24261), At TT19 27 (Q9FE46), Vv GST4 30 (Q56AY1), Pf GST1 54 (B1B5E6), Cs GST3 55 (I2FHU9), Dc GSTF2 56 (I4DUE3), Pp Riant 21 (M5WTI5), Lc GST4 57 (A0A0U4AVK2), Fv RAP 20 (UPI0002C2E8B9), Md GST1 25 (G3LX80), Ac GST1 26 (A0A2R6R622), Ib GSTF4 58 (A0A8E4DFU3), Cs GSTa 59 (A0A4P8P9K5), Pc GSTF12 60 (A0A6G5X592), Gt GST1 24 (NCBI accession number: BCD52748.1), Lh GST 23 (A0A5B9G8S8), Ep Bract1 61 (NCBI accession number: QTX16320.1), Gh GSTF12 22 (A0A5D2YJA6), Rs GST1 62 (A0A6J0K8F1), Pt GSTF8 39 (NCBI accession number, 5F07_A)Uniprot accession numbers unless stated otherwise) was constructed using Clustal Omega 1.2.2 63 with standard settings

Studies have shown that Drp1 is a core executor of fission, and its activity is regulated by phosphorylation: phosphorylation at Ser616 promotes mitochondrial recruitment and activates fission, while phosphorylation at Ser637 inhibits its activity (81, 82)