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Two primer pairs were employed for simultaneous amplification: tdTomato-tF1: 5-TACGGCATGGACGAGCTGTAC-3 Rabbit-PolyA-tR5: 5-CCAACCTTTGTTCATGGCAG-3 This primer pair amplified a 333 bp product specific to the B6-G/R knock-in allele, with no product detected in WT mice

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