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Ultimately, the goal is to remove every possible variable that isn't the one you're trying to test

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At 3 months of age, 85% of infants in the supplemented group had adequate vitamin B 12 blood levels compared to 36% in the placebo group.[25] One hundred twenty-four pregnant women in Canada received 12 mcg oral vitamin B 12 once daily beginning at 13 to 22 weeks gestation and continuing through 8 weeks postpartum

Albumin levels are low in many disease states, resulting in lower total drug concentrations