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The enzyme was dissolved in phosphate-buffered saline at pH 7.4, sterilized through a 0.22-m membrane, and administered under ultrasound guidance

Sequencing, data processing, and analysis parameters The study's primary aim was to determine whether 5A-1MQ treatment in combination with a switch from WD to LD rendered the intestinal microbiome comparable to that of mice continuously maintained on LD, and distinct from that of DIO mice switched to LD but given saline injections or mice maintained continuously on WD

In a properly functioning cycle, homocysteine is converted back to methionine through a process that requires vitamin B12 and folate