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Fourthly, Hepcidin starvation explains phenomenon that iron overload in patients with -thal who have not been treated with blood transfusion

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Sample Preparation: Fecal samples (50 mg) were extracted using ice-cold 80% methanol (500 L) containing internal standards, homogenized using bead beating (50 Hz, 3 min), vortexed for 20 min, and centrifuged (20,000 g, 4 C)

Moreover, scTS2/16 supports a six- to sevenfold increase in the yield of these organoids when cultured in collagen I hydrogels, both in 3D and 2D