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Blots were washed with PBS + 0.1% Tween 20, three times 5 min each, and probed with secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling #7074) at a concentration of 1:10,000 for 1 h at room temperature

The novel fungal CYP51 inhibitor VT-1598 is efficacious alone and in combination with liposomal amphotericin B in a murine model of cryptococcal meningitis

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Similarly, the spleen was minced to obtain a single-cell suspension, the cell suspension was added to Ficoll-Paque Plus at 400g for 30 min at room temperature (30)