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For analysis of flavonoids by HPLCMS, reaction volumes were split after the plate reader incubations, and a 40 l aliquot was extracted with 80 l of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid for quantification of cyanidin ( 8 ) concentration while a second 40 L aliquot was extracted with 80 l of 75% acetonitrile in H 2 O, 1 mM EDTA for quantification of all other flavonoids

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The Highly Sensitive DCFH-DA also has similar fluorescence characteristics (ex: 505 nm, em: 525 nm) to DCFH-DA

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Linear regression identified factors associated with elevated BA1 scores, while logistic regression determined factors associated with full AKI-R