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Isolation of mitochondrial and cytosolic extracts At the end of the culture time, cells were recovered by scrapping and collected by centrifugation at 2500 g for 5 min at 4 C

On the other hand, Central, Southern, and Western Europe had various daily Se intakes (3090 g/day), being the highest reported in France, Belgium, Spain, and lower in the UK, the Balkans, Austria, Hungary, Germany, and Italy (56, 57, 64)