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[6] Amplified NOS Activity - Glutathione stimulates total L-arginine turnover and increases NOS activity, resulting in greater NO production from available L-arginine

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In one aspect of the invention, one or more NNMT inhibitors (such as a cation of Formula I or IA, or otherwise disclosed herein) is contacted with a cell expressing NNMT concurrently with one or more chemical entities (e.g., nicotinamide riboside, nicotinamide mononucleotide) that increase intracellular NAD+ levels to provide muscular therapy

For analysis of disulfide bond formation by MALDI, the samples were desalted and concentrated using C18 Ziptip pipette tips (Millipore)