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Prospective evaluation of repeated supratherapeutic acetaminophen (paracetamol) ingestion

After rinsing the precipitant with EtOH (75%), it dissolved in DEPC-treated H 2 O

Following three washes in PBST (PBS containing 0.1% Tween-20), the sections were incubated with an anti-tyrosine hydroxylase (anti-TH) antibody, followed by a biotin-conjugated secondary antibody (Zsbio, Beijing, China