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Whereas ablation of a segment of the uterosacral ligament has not proved effective, randomized, controlled trials have shown the superiority of laparoscopic ablation of endometriotic tissue combined with presacral neurectomy (removal of the nerve bundle within the boundaries of the interiliac triangle) over laparoscopic ablation alone in improving dysmenorrhea and reducing severe midline pain (20)

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For flow cytometry analysis, cells were trypsinized and transferred to 1.5 mL tubes using a doubled volume of DMEM containing 10% FBS