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Analytical verification requires reverse-phase HPLC with UV detection at 214-220 nm to confirm retention time consistency and peak purity above 95%, while mass spectrometry (MALDI-TOF or ESI-MS) can confirm the expected molecular ion at 1419.53 Da and identify any truncation or oxidation products

These findings suggest that insufficient and prolonged sleep durations are associated with elevated microglial density in frontostriatal and limbic systems, respectively, among healthy middle-aged and older adults, without any associations with peripheral inflammation

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