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Collagen gel contractility is enhanced in human endometriotic stromal cells: A possible mechanism underlying the pathogenesis of endometriosis-associated fibrosis

If you are alone, the outer- anterior thigh is likely the easiest way to administer the injection but may cause more discomfort than the upper buttocks

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Tissue sections were incubated with 0.3% Triton X-100 (Sigma-Aldrich), 5% normal goat serum (Jackson ImmunoResearch Inc.), 2% ovalbumin (Sigma-Aldrich) in phosphate buffered saline for 3 h at room temperature (RT) to block non-specific binding of antibodies