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Store between 15-25C and keep in a cool place

Lysates were cleared using QIAshredder spin columns (Qiagen), boiled in Laemmli buffer and resolved on NuPAGE 412% polyacrylamide gels (Invitrogen)

Moisture can cause hydrolysis and clumping

The crude material was purified by Isolera flash column chromatography on silica via gradient elution, from 100% dichloromethane to 15% methanol in dichloromethane to produce CAM-S-CAM-TPP (9.1 mg, 16%) as a pale yellow fluffy/foamy solid