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Functional analysis of genetic variation in catechol-O-methyltransferase (Comt): Effects on mRNA, protein, and enzyme activity in postmortem human brain

Mitin N, Rossman KL, Der CJ

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Lysates were treated with Na 2 S 2 or Na 2 S, followed by alkylation with IAM-TPP or IAM (procedures A or B, respectively), and then adsorbed onto mixed hydrophobic/hydrophilic solid matrix SP3 beads to facilitate processing of the protein sample without precipitation and resuspension 48,49 (Supplementary Fig