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Temporal Considerations: Acute effects (hours-days): NAD+ elevation, initial sirtuin activation Chronic effects (weeks): Mitochondrial biogenesis, metabolic improvements, body composition 4

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MFI means the amount of weight at the moment when the rat, held upright with the hind limb extended and placed upon a digital scale precision 0.001 g, starts to bear weight on the scale, with normal (N) or experimental (E) injured leg, providing equation MFI = (NEPT EEPT)/NEPT, and MFI maximal value of l as the worst scenario [21]

Light Protection: Keep vials in their original box or a dark drawer