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Angiogenic 3-Dimensional Co-Culture Assay HUVEC (Lonza) cells were transfected with lentiviral GFP and human brain derived pericytes (Sciencell) were transfected with lentiviral Red Fluorescent Protein (dsRED), according to the manufacturers instructions (Dharmacon)

DTT treatment increases rips-1 transcript abundance We next used qRT-PCR to assess the gene expression changes after exposure of synchronised wild-type N2 L4s to 5 mM DTT for 24 h, conditions we found to have a minimal effect on worm development within this time frame

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Local TH activation is crucial for UCP1 expression and consequently adaptive thermogenesis, and its impairment despite normal serum TH levels could suggest reduced thermogenic capacity or function under prolonged stress [29, 30]