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Reconstitution of CHAC1 or MitoCHAC1 in CHAC1-knockout cells involved cloning of the respective nucleotide sequences purchased as gBlocks from Integrated DNA Technologies into the pLenti-CMV-GFP-Hygro vector (Addgene, 17446)

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Comprehensive proteomic analysis was performed using nano liquid chromatography tandem-mass spectrometry

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