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Among the 122 proteins downregulated in PQS-treated shCNMT RAW264.7 macrophages, the iron uptakeassociated single-pass membrane receptor TFR1 was directly related to ferroptosis as the critical downstream effector of CNMT 15

By inhibiting NNMT, 5-Amino-1MQ reduces 1-MNA formation and alleviates the methyl sink, increasing SAM availability for other methylation reactions, while freeing nicotinamide for recycling through NAMPT toward NAD

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Its a powerful antioxidant made up of three key amino acids: glutamate, glycine, and cysteine

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