glutathion sythetase Metabolic Synthesis of Clickable Glutathione for Chemoselective Detection of Glutathionylation Glutathione synthesis: A two-step pathway.

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10.1097/00003086-199704000-00029 [DOI] [PubMed] [Google Scholar] Keremi B., Lohinai Z., Komora P., Duhaj S., Borsi K., Jobbagy-Ovari G., et al

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Chronic stress and inflammatory stimuli promote microglial activation, leading to sustained release of pro-inflammatory cytokines, reactive oxygen and nitrogen species, and excitotoxic mediators that disrupt synaptic function and neuronal integrity (30)

Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C