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Beads were subsequently washed with 10 column volumes (cv) of 1x150 mM NaCl, 5 mM EDTA in 50 mM Tris, 1x150 mM NaCl in 50 mM Tris, 1x450 mM NaCl in 50 mM Tris and 50 mM Tris buffer without salt

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Assessing the risk of antibiotic resistance transmission from the environment to humans: non-direct proportionality between abundance and risk