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Tirone, P.D

GraphPad Prism 9 (Version 9.5.1) (GraphPad Software, Boston, MA) was used for statistical analyses

Next, we directly assessed whether the PVN Glu neurons contribute to these tonic currents in the DMV Ach neurons, specifically by ablating PVN Glu neurons based on infusion of a Cre-dependent genetically engineered caspase3 (AAV-DIO-taCaspase3) and AAV-DIO-EGFP viruses (or AAV-DIO-EGFP as a control) into the PVN, as well as FG injection into the small intestine of VgluT2-Cre mice (Supplementary Fig