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One theory is that due to the association between increased abundance of hybrid Insulin/IGF-1 receptors on target human tissues and elevated plasma insulin observed in patients with hyperinsulinemia, that these hybrid IR receptors may cause insulin resistance in certain human tissues

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Chen Q, Chao Y, Zhang W, Zhang Y, Bi Y, Fu Y, et al

After collecting the supernatant containing the virus and calculating the virus titre, the cells were infected with 2 mL of the viral supernatant, followed by puromycin (2 g/mL) selection for 2 weeks

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