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Dead cells were excluded with Dead Cell Removal Kit (NovoBiotechnology Co., Ltd., Beijing, China)

The influence of glycogen synthase kinase 3 in limiting cell addition in the mammalian ear

Similarly, no marked abnormalities in these parameters were detected or observed (Supplementary Figure S11 and S12)

Arsenic exposure has been shown to significantly elevate intracellular ROS levels, and an excess of ROS can cause oxidative damage to DNA, resulting in modifications to DNA bases and double-strand breaks