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Electrophysiological recordings demonstrate that Dihexa-treated neurons exhibit significantly increased frequency of miniature excitatory postsynaptic currents (mEPSCs), indicating enhanced synaptic transmission

Nonetheless, multiple sources of bias likely influenced results, including exposure misclassification (es., current shift status used as proxy for lifetime exposure), survivor bias and left truncation in cohorts, and incomplete control for reproductive or behavioral confounders 276

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What to look for in a source Third-party testing: Reputable vendors provide certificates of analysis from independent laboratories confirming purity, typically 98%+ for research-grade peptides

Materials and methods Reagents and antibodies Antibodies used in Western Blot (WB) and flow cytometry (FC) are listed in Table S1 and analytical methods in Supplementary Materials and Methods (SMM)