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Physical characterization was performed via particle analyzer/zetasizer, electron microscopy [SEM/TEM] analysis and protein identification, quantification was performed by Western blot analysis for known EV markers of lysed EVs

meningitidis strains in vitro using the double-strain biofilm system, despite the fact that they produce the fratricide toxins TpsA and MafB (Prez-Ortega et al., 2017), although it can be noted that colonization of the nasopharynx by different meningococcal strains is very rare and detected only in approximately 1% of carriers (Caugant et al., 2007)

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Sequence and Phylogenetic analysis of DJ-1/PfpI proteins The maximum likelihood (ML) tree plotted using all the 217 sequences showed four major clades